Crowds chanted, “Peacock, Peacock” in celebration after the 19-year-old world record holder smashed his own record to finish in 10.90 seconds in the T44 100m on Thursday evening.
American Richard Browne finished second, with South Africa’s Arnu Fourie third and Oscar Pistorius fourth.
Pistorius, who won silver in the 200m and gold in the 4x100m, paid tribute to Peacock’s performance, describing his victory as “one of the great Paralympic performances”.
Peacock, who lost his right leg below the knee after contracting meningococcal septicaemia at the age of five, said his victory in front of a packed crowd at the Olympic Stadium would live with him forever.
“This is absolutely amazing,” the Lee Valley-based athlete from Cambridge told BBC Radio 5 live. “I’d like to have run a little bit quicker, but I did enough to hold on.
“To hear the crowd chant my name was amazing. That’s going to live with me forever. I could not have wished for better.
“I crossed the line thinking ‘have I won?’ I waited for the results to come on the board. It was such a relief. There has been such a big build-up over the past year. This is immense.”
Peacock shot to prominence in 2010 when, as a 16-year-old, he recorded a personal best of 12.23 seconds in his first Paralympic World Cup race in Manchester.
Since then he has broken the European record and gone on to break 2004 Paralympic champion Marlon Shirley’s world record.
His Thursday evening performance created one of the moments – if not the moment – of the Games, turning the tables on Pistorius and crowning his glorious rise with Paralympic gold.